STUDY OF ANTIMICROBIAL ACTIVITY OF LEAVES AND FRUIT SKIN OF BLACK YEMENI GRAPE

الملخص

Grape is one of the oldest fruits which consumed by human, and described as one of the three kings of fruits (grapes, dates and figs). This fruit has many uses, nutrition and therapeutic. The aim of this study was investigation the antimicrobial activity of leaves and skins extracts of one type of black Yemeni grapes. These extracts were used against 16 types of disease-causing bacteria and 8 types of skin disease-causing yeasts. The results showed that ethanol extract of grape skins had a good effect against all Gram positive bacteria tested in this study, and the maximum zone of inhibition was 20 mm against Streptococcus pyogenes, 19mm with MIC 12.5μg/ml. Also, strong antimicrobial activity was obtained against Gram negative bacteria and the maximum zone of inhibition was 18mm against Yersinia enterocolitic with MIC 12.5μg/ml, this extract also has excellent activity against yeasts. The ethanol extracts of leaves of grapes showed inhibition growth of most tested microorganisms with various degrees of inhibition. The results found that the grape extract was exhibited high antimicrobial activities against all tested Gram positive bacteria (12.5μg/ml), also was effective against 63% of Gram negative bacteria and the maximum inhibition zone diameter of this extract was 17 mm against Klebsiella pneumoniae with MIC 25μg/ml.