EFFECT OF MIXTURE OF SWEET RED BELL PEPPER AND VIRIGIN OLIVE OIL ON MANY BIOCHEMICAL AND HISTOLIGICAL PARAMRARS ON BANCREASE OF DIABETIC MALE RATS

Abstract

The recent study aimed to evaluate the synergistic effect of mixed sweet bell pepper extract Capsicum annuum L. and virgin olive oil Olea europaea L. in many biochemical and physiological parameters, with histological study of pancreatic organ on albino male rats exposed to oxidative stress induced by alloxan, Twenty adult male rat has been used in experiment with age (12-14) week with weight (250-300) gram, the water and standard food gave them ad libitum in animal house belong to science college , the result shown as bellow: Male rats treated with alloxan has been shown high significant increasing at level (P<0.01)Nin total cholesterol, Triglyceride, Low density of lipoprotein in compare with control group. Whereas high significant decreasing in HDL-C in compare with control croup. The treatment of animals exposed to oxidative stress via alloxan with mixed of Capsicum annuum L. and virigin olive oil have been shown significant decrease at level (P<0.01) in TC , TG, LDL-C concentration ,while the mixed has strong effect on increasing of HDL-C compare with alloxan group. the giving of the mixed extracts which are Capsicum annuum L. Olea europaea L. for animals exposed to alloxan has caused high significant decrease (P<0.01) in Glucose concentration and high significant increase in Insulin Conc. compare with alloxan group and alloxan with red bell pepper. As diabetic animals which are treated with mixed of sweet bell pepper and olive oil has show high significant decrease in ALT & AST activity compared with them level in diabetic’s rats, whereas AST activity did not show any differ in diabetics animal treated with red bell pepper alone. The mixture of sweet bell pepper and olive oil has revealed high power in decrease of MDA conc. In experimental diabetics rats and increasing in glutathione conc. Compared with diabetics group treated with alloxan. Histological study for pancreas tissue of animals group exposed to alloxan many damaged and cytoplasm necrosis of Langerhans islets, facular necrosis, many of big cells reached to apoptosis stage addition to hemorrhage and congestion, while treatment of diabetics animals with mixed extract caused in improvement in hole parts of tissue, Langerhans cells appear normal and there has not an hemorrhage.